Assessment of lipid peroxidation and serum electrolyte profile in epileptic patients exposed on anti-epileptic drugs

Background: Epilepsy is one of the most common severe disorders of the brain and 50 million people are affected by epilepsy from all over the world. In the central nervous system [CNS] electrolyte homeostasis is crucial for the brain function. Anti-Epileptic Drugs [AEDs] are neither protective nor healing but employed exclusively to control symptoms such as suppression of seizures

Objective: To assess lipid peroxidation and serum electrolyte profile in epileptic patients exposed on anti-epileptic drugs

Methodology: Fifty patients of epilepsy and ten age and sex-matched clinically apparently healthy individuals were eligible for inclusion in the study at Jinnah Hospital Lahore. 5.0 ml blood sample was taken from each individual and subjected to centrifuge at 3000-4000 rpm for 10-15 minutes for the separation of serum. The estimation of anti-oxidative biomarkers [SOD, MDA, GSH and CAT] and serum electrolyte profile [NA+, K+, Ca++ and Mg++] were estimated

Results: The level of MDA in epileptic patients was increased remarkably [7.08 +/- 0.76] as compared to healthy individuals [1.67 +/- 0.19] and statistically significant [0.000<0.05]. Mg++ level was decreased in epileptic patients [1.44 +/- 0.21] as compared to control [1.68 +/- 0.20] and statistically significant [0.003<0.05]. Serum sodium [Na+] level in epileptic patients was increased [1.64 +/- 9.9] as compared to control persons [1.38 +/- 5.97] and statistically significant [0.004<0.05]

Conclusion: A relationship is present between oxidative stress, electrolyte profile and epilepsy. Lipid peroxidation level and sodium and potassium level is significantly elevated in epileptic patients as compared to control persons

Assessment of biochemical and antioxidative status in patients suffering from dengue fever / 华中科技大学学报（医学）（英德文版）

A multi-centred study was designed to collect dengue epidemiologic data from government and registered private hospitals/clinics and maintained archive of frozen specimens in bio-bank to be used for future dengue epidemic control program, and assess the epidemiology of dengue fever (DF) by evaluating biochemical and oxidative status of patients. ELISA IgM antibodies test was done to confirm DF. From August 2010 to December 2011, 101 confirmed blood samples of DF patients referred to pathology lab of Jinnah Hospital Lahore were subjected to the epidemiologic assessment by evaluating the biochemical and physiological indices and alterations of circulating antioxidants. Clinical features of DF patients and effect of fever on blood components and serum proteins of liver were recorded. The hospital stay in DF, dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS) showed significant difference. Significant increases in serum alanine amino transferase (ALT) (P=0.000), aspartate amino transferase (AST) (P=0.000), alkaline phosphatase (ALP) (P=0.000), malondialdehyde (MDA) along with significant decreases in total protein (TP) (P=0.000), reduced glutathione (GSH) (P=0.000), superoxide dismutase (SOD), catalase (CAT) (P=0.000), and sialic acid contents (P=0.016) were observed. A positive correlation existed between bound sialic acid levels, liver enzymes and circulating antioxidants (r=0.656, P=0.016). In the present study, alterations of circulating antioxidants in DF suggest that DF might be a metabolic response to an acute, self-limiting tropical viral infection, and a consequence of the viral inflammatory process.

Significance of hepatic profile and malondialdehyde as marker of lipid peroxidation in HCV patients: a perspective study from local population of Punjab

All over the world Hepatitis C virus [HCV] remains to be a main etiological mediator of liver disease. Approximately, 10 million people in Pakistan are diseased with HCV. Pegylated interferon plus ribavirin signifies the gold standard therapy but various side effects may occur. Comparative study. This study was conducted at Jinnah Hospital Lahore from August 2013 to March, 2014. Thirty five patients of hepatitis C virus and Twenty three age and sex-matched clinically apparently healthy individuals were eligible for inclusion in the study at Jinnah Hospital Lahore during the year 2013-2014. 1.0 ml blood sample were taken and subjected to centrifuge at 3000-4000 rpm for 10-15 minutes for the separation of serum. All the analytical work was performed at the Institute of molecular biology and biotechnology [IMBB], and Centre for research in molecular medicine [CRiMM], The University of Lahore-Pakistan. The estimation of AST, ALT, ALP, TP and T.Bilirubin were estimated. The AST level in HCV patients was increases [47.88 +/- 40.49] as compared to the control persons [31.43 +/- 7.31] and statistically significant [0.02<0.05]. Total Protein level in HCV patients was [4.20 +/- 0.61] and in healthy individuals [6.23 +/- 0.51] and statistically significant [0.000<0.05]. MDA level in HCV patients was increases remarkably [8.58 +/- 1.19] and in control persons [1.47 +/- 0.54] and it was statistically significant [0.000<0.05]. There is a relationship between oxidative stress and ALP, ALT, AST and Albumin. The results of the present study confirmed a perfect sketch regarding the circulating biochemical markers and lipid peroxidation [MDA] profile between the studied groups i.e., control and HCV patients with interferon induced Hepatitis C virus infection